Pioneering Plasmid-IQ™ Design: Accelerating Cell And Gene Therapy For Faster And More Efficient Solutions

Plasmids are essential raw materials in the production of recombinant proteins and viral vectors. Despite their limited direct use in humans due to immune reactivity and delivery inefficiencies, plasmid DNA plays a critical role in manufacturing viral vectors such as adenoviruses, lentiviruses, and adeno-associated viruses (AAV), where its application has been extensively optimized for gene delivery.

Our Plasmid-IQ concept is revolutionizing plasmid engineering by focusing on the proper development, refinement, and application of these genetic elements to ensure manufacturing processes are done right the first time. By leveraging scientifically proven design principles, Plasmid-IQ eliminates sequence inefficiencies and fine-tunes plasmid architecture to enhance performance. This leads to improved gene expression, increased vector yields, and reduced production risks.

In this white paper, our experts provide insights into the significance of plasmid design in viral vector production, the value of establishing a Plasmid Master Cell Bank (MCB), and the use of analytical assays and Next-Generation Sequencing (NGS) for plasmid verification. Additionally, the paper explores methods for colony screening to ensure plasmid integrity and eliminate plasmid dimers.