By Kevin Khovananth, Tanya Knaide, Keith Albert, Artel
An integral, yet often overlooked, procedure with any given assay, washing steps are vital to ensure reaction quality and integrity particularly when conducted in a microtiter plate. Whether it is complete removal of unbound material within an ELISA, effective evacuation of residual wash buffer solution, or even simple removal of supernatant such as cell media just above the surface of adherent cells, effective washes can make a significant difference in the final results.
Here, we demonstrate the ability of the MVS® Multichannel Verification System to test the performance of an automated plate washer. This MVS method of performance measurement generates more inclusive and informative output reports that present data in a well-by-well manner for ease of visualization and discovery.