Overcoming Bispecific Antibody Purification Challenges With CHT™ Ceramic Hydroxyapatite Media

Bispecific antibodies can present unique downstream purification challenges, including aggregation and the removal of product- and process-related impurities. This application note examines CHT™ Ceramic Hydroxyapatite Media as a polishing strategy for asymmetric and symmetric bispecific antibodies. Across three model molecules, CHT Media achieved high aggregate removal and at least 97% purity, while reducing chromatography-induced aggregation compared with a traditional cation exchange approach. Explore the study results and how multimodal chromatography can help address challenging bispecific antibody separations.