Next Steps In AAV Characterization Via Light Scattering Measuring AAV Critical Quality Attributes

By Anatolii Purchel, Ph.D., Michelle Chen, Ph.D. and John Champagne, Ph.D.

Adeno-associated virus (AAV) AAV has mild immune response, able to deliver its payload to a wide range of host cells, and is becoming a prominent delivery vehicle in gene therapy.

Multi-Angle Light Scattering (MALS) determines absolute molar mass and size directly, in solution, independent of elution time and conformation. This technique, combined with multiple concentration detectors allows independent protein and nucleic acid payload characterization.

In this example the SEC-MALS method is presented for measuring total capsid particle concentration, relative capsid content, and percentage of monomer or aggregates. Field-flow fractionation and batch DLS were employed to further characterize amount of aggregates and particle concentration.

This poster examines how the SEC-UV-MALS-dRI measures AAV particle concentration, relative capsid content, and aggregation all in a single run