Multi-Column Chromatography For Efficient Polishing Purification

Continuous bioprocessing demands efficient downstream operations, and Multi-Column Chromatography (MCC) offers a powerful solution for polishing steps. By applying MCC with cation exchange resins in bind/elute mode, manufacturers can achieve high selectivity even when separating proteins with similar physiochemical properties. Studies show that MCC doubles productivity compared to batch purification while reducing resin requirements from 30 L to just 2.4 L. Automation through systems like Mobius® Multi Column Capture minimizes operator intervention and ensures consistent performance across cycles. With purity levels reaching 82% and recovery at 92%, MCC enables cost savings, process intensification, and a smoother transition to fully continuous manufacturing.

Explore how this approach can optimize your downstream workflow and overcome traditional bottlenecks.