Measuring Physical Properties Of Liposomes And LNPs For RNA Delivery With MD-AF4

Multi-detector asymmetric-flow field-flow fractionation (MD-AF4) has been recognized as an invaluable tool for the characterization of particle size, polydispersity, drug loading and stability of nanopharmaceuticals. The application of robust and high-quality standard operating procedures (SOPs) is critical for accurate and reliable measurements, especially as these complex drug nano-formulations are most often inherently polydisperse.

After a brief overview of MD-AF4 principles and instrumentation, this webinar presents examples of how MD-AF4 quantifies key quality attributes of complex nanomedicine formulations such as particle size distributions, shape, concentration and stability, using the SOP guidelines. Particular focus is given to the characterization of liposomal products and of lipid-based nanoparticles (LNPs) for RNA delivery. Finally, current efforts within ASTM for standardization of methods based on MD-AF4 are presented.