mbDNA™: The Smarter DNA For Non-Viral Gene Editing

Get introduced to a novel circular single‑stranded DNA platform designed to overcome long‑standing challenges in non‑viral gene editing. Traditional viral vectors face payload constraints, immunogenicity, and complex manufacturing, while common double‑stranded DNA donors can trigger cellular stress and reduce editing efficiency. Earlier single‑stranded templates offered lower toxicity but were limited in length and stability. This new DNA format combines a customizable double‑stranded stem with a fully user‑defined sequence of up to 20 kb, enabling efficient homology‑directed repair across diverse cell types. Its architecture supports high cell viability, robust knock‑in and knock‑out rates, and compatibility with multiple delivery modalities, including electroporation and nanoparticle‑based systems. Data show consistently strong performance in primary immune and stem cells, maintaining healthy expansion and producing high integrative efficiency even at scale. With its stability, reduced innate immune activation, and versatile design, this platform represents a significant advance in non‑viral gene editing and emerging therapeutic applications.