mbDNA™ + Custom Circles: Touchlight's CircularDNA Architectures For Gene Editing

Overcoming the payload, toxicity, and stability limitations of traditional gene editing requires a departure from legacy DNA molecules. Next-generation circular DNA architectures—including unique single-stranded, hybrid, and double-stranded circular configurations—are purpose-built to enhance cell viability and reduce immunogenicity. By utilizing a single-stranded nature or excluding traditional backbone sequences, these constructs significantly minimize activation of innate immune pathways like cGAS-STING, unlocking safer options for both immune cell engineering and systemic, lipid-formulated in vivo applications.

Produced via an advanced enzymatic cell-free platform, these architectures deliver exceptional purity, minimal donor-to-donor variability, and an expanded payload capacity capable of carrying large genetic cargos up to 20kb. In real-world applications, these unique formats consistently achieve outstanding knock-in rates of 60% to 75% in primary human T cells while maintaining superior cell survival. Review the complete technical info sheet to discover how custom circular DNA formats can optimize your homology-directed repair (HDR), transposase, and episomal expression workflows.