Harvest Of HEK Cells In Extracellular Expression Systems And For n-1 Seed Train Intensification

Evaluate the performance of CARR Biosystems’^® UFMini single-use centrifuge in the clarification of Expi293F cells by measuring supernatant turbidity and viable cell recovery (VCR). The CARR Biosystem’s UFMini single-use centrifuge has been shown to successfully purify and concentrate the Expi293F cell feed while exhibiting low shear stress with minimal impact to cell viability.

This demonstration shows that the UFMini can be utilized to optimize clarification processing efficiency by providing a user-friendly single-use centrifugation process that maintains cell viability and produces a highly clarified effluent that can help to reduce burden on downstream processes.