LC-MS/MS Method Development Of Endogenous Small Molecule Biomarker

Biomarkers — endogenous molecules that indicate drug safety, mechanism of action, efficacy, and disease state progression — are essential to improve drug R&D efficiency. Bioanalytical scientists must overcome challenges to develop a bioanalytical method to meet regulated bioanalysis criteria.

This poster provides several cases to highlight some practical solutions, including the roles of matrix choice without interference, background subtraction, chromatographic optimization, sample handling, and sample preparation for achieving a fit-for-purpose LC-MS/MS biomarker analysis method.