Large Scale Purification Of Oligonucleotides With Ion Exchange Chromatography

The growing prominence of oligonucleotide-based therapeutics, such as siRNA and antisense DNA, underscores the necessity for efficient purification methods to support drug development and production. Producing oligonucleotides at a clinical scale presents challenges, particularly in achieving high purity and yield. Ion exchange chromatography (IEX) offers a robust solution for oligonucleotide purification, combining scalability, efficiency, and environmental sustainability.

This study explores the innovative approach by BianoGMP GmbH to purify oligo(dT) using the AZURA® preparative HPLC system. Employing strong anion exchange resins, the team achieved a remarkable 95% purity and 90% yield, meeting the stringent demands of therapeutic applications. The results highlight the importance of IEX in overcoming upscaling barriers while maintaining exceptional quality standards. Discover how advancements in IEX technology are shaping the future of oligonucleotide manufacturing.

Access the full content to dive deeper into methodologies and real-world applications of IEX in pharmaceutical innovation.