Chapter Three: Key Requirements For Efficient And Linear Scale-Up Of Shear Sensitive Cell Therapy Products Using The Vertical-Wheel Bioreactor

In the third chapter of this webinar series, discover how scaling up medium exchange for pluripotent stem cell (PSC) aggregate cultures presents significant challenges, particularly with traditional settlement-based methods that lead to aggregate heterogeneity and compromised culture quality at larger volumes. The implementation of a perfusion-based medium exchange system successfully addressed these issues by maintaining a stable microenvironment and enabling consistent scalability across bioreactor sizes, from PBS 0.1L to PBS 3L. Learn how oxygenation, another critical hurdle in scale-up, was resolved through increased agitation and external oxygenation to preserve cell integrity and growth.

Now is the time to adopt perfusion-based strategies and optimized oxygenation techniques to ensure reproducible, high-quality results in large-scale bioprocessing.