Chapter Four: Key Requirements For Efficient And Linear Scale-Up Of Shear Sensitive Cell Therapy Products Using The Vertical-Wheel Bioreactor

In the fourth chapter of this webinar series, gain a comprehensive overview of a successful strategy for scaling pluripotent stem cell (PSC) cultures using a vertical wheel bioreactor. The team demonstrates linear scalability from 3-liter to 80-liter volumes while maintaining consistent cell growth rates and aggregate quality. Flow cytometry confirmed the preservation of key pluripotency markers (SSCA4 and TRA160) across all scales. To support this scalability, they developed low-shear, efficient methods for harvesting, washing, and concentrating cells, including a centrifugation-based device and an in-vessel dissociation technique for aggregate cultures—achieving high recovery and viability rates. The platform integrates 2D and 3D expansion stages, differentiation processes, and post-expansion handling, which makes it suitable for a wide range of cell types and applications in cell and gene therapy, cellular agriculture, and clean meat.

If you're looking to scale your cell therapy processes with precision and efficiency, now is the time to explore how this platform can transform your manufacturing pipeline.