Improving The Enrichment Of Mononuclear Cells For CAR T Cell Therapies: The Importance Of Platelet Depletion

Enriching mononuclear cells (MNCs) by removing other cell types and contaminants is a critical step in CAR T cell therapy production. This video explains why, how, and when you should carry out platelet depletion to achieve successful T cell enrichment.

Highlights:

• Optimal MNC enrichment is critical to the successful start of a standard autologous CAR T manufacturing process.
• A platelet removal step upstream rather than downstream of density gradient separation can reduce red blood cell contamination.
• Working with a closed and automated system minimizes the risk of contamination, operator variability, and errors.