How To Fingerprint Cell Culture Media

By Jude Lakbub, MilliporeSigma

Quantifying individual components of cell culture media prior to batch release is essential for maintaining media identity and consistency in biopharmaceutical manufacturing. Given the complexity of these formulations, comprehensive media identification remains a significant challenge. Cell culture media (CCM) are foundational to the upstream production of biologic drugs, supplying the necessary nutrients, growth factors, and environmental conditions that support optimal cell growth and productivity. The precise composition of the media directly influences cell viability, recombinant protein expression, and critical quality attributes of the biologic product, such as glycosylation. Therefore, consistent and well-optimized media formulations are vital for achieving high yields, ensuring batch-to-batch reproducibility, and meeting regulatory standards.

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