How Can I Increase My Viral Titers And Improve The Percentage Of Full AAVs?

Optimizing viral vector production involves several strategies, ranging from simple reagent changes to complex platform shifts. This article discusses variables that can be adjusted to increase viral titer and modulate the percentage of full AAV capsids.

Transfection reagents, cell lines, and cell culture media are key factors. Using specialized reagents and cell lines can significantly improve viral yield, while optimizing transfection procedures and DNA constructs can further enhance results. The most dramatic change lies in the cell culture platform. Scaling up to larger systems can increase overall viral vector yield, but requires careful consideration of compatibility with existing processes.

By exploring these strategies and considering the specific needs of your research, you can effectively improve viral titer and achieve higher-quality viral vectors. To learn more about these strategies and their implementation, please read the full article.