Holographic Imaging Of Insect Cell Cultures: Online Non-Invasive Monitoring Of Adeno-Associated Virus Production And Cell Concentration

This work shows that digital holographic microscopy (DHM) can be implemented for online monitoring of Sf9 concentration and viability, as well as for monitoring product titer (AAV) or culture progression in lytic systems. Therefore, it is a valuable tool to support the time of harvest decision and for establishing controlled feeding strategies.

The insect cell-baculovirus vector system has become one of the most popular platforms for viral vector expression for vaccination and gene therapy purposes. Since it is a lytic system, it is essential to balance maximum recombinant product expression with harvest time, minimizing product exposure to detrimental proteases. With this purpose, new bioprocess monitoring solutions are needed to accurately estimate culture progression. Analyze how online DHM monitored bioreactor cultures of Sf9 insect cells.