High Throughput AAV Viral Titering Using Nanoplate-Based Digital PCR

As gene therapies move from innovation to commercialization, speed, accuracy, and reliability in process analytics are paramount, especially when it comes to viral vector quantification. Traditional digital droplet PCR (ddPCR) methods, while reliable, are often hindered by long run times, matrix sensitivity, and workflow complexity. Authors Kelly Geosits and Brian Tomkowicz introduce a powerful alternative: nanoplate-based digital PCR (dPCR) using Qiagen’s QIAcuity Digital PCR System.

With significantly reduced run times (as short as two hours), enhanced multiplexing capabilities, and compatibility with a wide variety of sample matrices, the QIAcuity system delivers precise and reproducible titers of AAV samples across all stages of development. Head-to-head comparisons show that QIAcuity delivers similar accuracy while offering faster results and higher throughput, leading to better data, quicker decisions, and reduced risk of costly delays.

Whether you're working with bulk harvest or polished AAV samples, this streamlined approach helps ensure your gene therapy programs meet regulatory and clinical expectations. Discover how nanoplate-based dPCR can accelerate your path to market.