Go Bigger, Stay On Target, And Develop The Cell Lines That Get The Job Done: TARGATT™ Large Knock-in Technology

Although CRISPR has transformed the life sciences and enabled groundbreaking advances in gene editing, it is not always the most suitable tool for every application. The technology relies heavily on host DNA repair pathways, which introduces variability and limits its effectiveness in certain contexts.

For example, knock-in efficiency drops sharply when inserting DNA fragments larger than 3–5 kilobases, creating significant challenges for projects that require the integration of large genetic elements. In addition, the induction of double-strand breaks (DSBs) — a core mechanism of CRISPR editing — carries inherent risks, including unintended off-target modifications and genomic instability. These factors make CRISPR less ideal for mammalian cell engineering projects where large knock-ins, precise control, and high safety standards are essential.