Femtobiomed Inc. Cell & Gene Tech Expo August 2025: Gene Therapy

The transition to non-viral vectors is critical for the future of cell and gene therapy, but conventional electroporation methods present a significant roadblock. The process introduces two forms of stress: intense thermal damage from Joule heating and electrical damage to sensitive subcellular organelles. This damage compromises cell health and limits post-transfection proliferation, a critical factor for successful gene editing outcomes, particularly for complex knock-in applications.

A dual approach can overcome these limitations. By pairing the CellShot® PFEP™ system, which uses a microfluidic design to completely dissipate thermal energy , with the Proliva™ buffer, designed to protect organelles from electrical stress, it is possible to maintain cell viability and function. This strategy dramatically improves post-electroporation health, increasing proliferation rates in some cells from ~60% to over 90%. Discover the data behind the CellShot PFEP™ and Proliva™ solution in this full presentation.