By Sandy McNorton, Thibaut Deschamps, Nathan Stegemann, Kim Schrag, Kate Achtien and Charlotte Javalet
A chemically defined medium specialty formulated to get the best performances for Spodoptera frugiperda (Sf) cells
The Baculovirus Expression Vector System (BEVS) is a powerful eucaryotic vector system used to produce viral vaccines and gene therapy vectors. Spodoptera frugiperda (Sf) cell lines are widely used as hosts for BEVS. However, FDA’s Retrovirus Laboratory discovered that the majority of Sf cells (such as Sf9) contain an unknown Sf-rhabdovirus that is now considered a process contaminant and must be eliminated during the bioprocessing (Hailun Ma et al. 2014). Because viral safety is essential in the manufacture of biopharmaceuticals and required to ensure patient safety, we offer a proven Sf9-rhabdovirus-negative (Sf-RVN®) Insect Cell Line that improves the safety profile of our customers’ bioprocesses. In order to get excellent growth and productivity of the Sf-RVN® Insect Cell Line, we specifically developed a chemically defined medium: the EX-CELL® CD Insect Cell Medium. Combined, these two products form the Sf-RVN® Platform and provide a high performant rhabdovirus-free BEVS alternative to produce recombinant proteins, Virus-Like Particles (VLP) for viral vaccines, and Adeno-Associated Viruses (AAV) to treat genetic diseases.
In this study, we explored the performances of the EX-CELL® CD Insect Cell Medium. We compared the growth of two Spodoptera frugiperda (Sf) cells, our rhabdovirus-free Sf-RVN® Insect Cell Line and its parent, the Sf9 cell line, both cultivated in 6 different cell culture media.