Enhancing Preclinical Research Precision: Analyzing CAR-T Biodistribution With Digital PCR (dPCR)

In the dynamic landscape of gene and cell therapies (CGTs), an accurate understanding of cell product biodistribution is pivotal to monitoring safety and efficacy. This requirement can be met by the emergence of digital polymerase chain reaction (dPCR), a technology enabling precise DNA quantification. The heightened sensitivity of dPCR over other technologies distinguishes target DNA from the genomic background, which is crucial for efficiently monitoring biodistribution in animal models. Our presentation introduces a dPCR workflow tailored for assessing the biodistribution of human CAR-T therapy and leukemia cell lines in rodents. This innovative approach showcases our dPCR workflow's potential to optimize precision and reliability, setting a benchmark for preclinical assessment of investigational cell therapies.