Enhancing Non-Viral CAR T Manufacturing With MaxCyte Electroporation And Akadeum Microbubble T Cell Isolation

Non-viral engineering workflows demand both a robust electroporation platform and high-quality upstream cell populations for reliable outcomes. Discover how combining clinically validated electroporation with gentle, microbubble-based cell isolation can significantly strengthen your engineered T cell workflows.

The integration of microbubble isolation upstream of the electroporation process has been shown to improve critical post-engineering outcomes. We highlight experiments using DNA, mRNA, and a representative CRISPR-based CD19 CAR knock-in that demonstrated superior results compared to conventional magnetic separation methods, including:

• Higher viable cell numbers
• Greater cell viability
• Superior transfection efficiency

Gain practical insights into how this combined approach can deliver more reproducible results, streamline manufacturing, and ultimately accelerate the advancement of life-changing engineered T cell therapies. Watch the on-demand webinar now to learn how to optimize your cell isolation and engineering steps.