Empty/Full Capsid Ratio Of AAV By Sedimentation Velocity – Analytical Ultracentrifugation

Empty adeno-associated virus (AAV) capsids have the potential to impact safety and efficacy, since they may stimulate an unwanted immune response without delivering the intended therapeutic benefit. It is important to characterize the relative amounts of empty and filled capsids within an AAV product. 

The results detailed here were generated from an internal study using AAV8 capsids and shows the boundary data for a mixture of empty and filled AAV8 capsids and displays the evolution of two visibly distinct boundaries during the experiment.