By Sofia Pezoa, Randall Alfano, Atherly Pennybaker, Nathan Hazi
In this poster we explore the optimization of parameters for lentivirus production in serum-free medium flatware. To determine if a serum-free, blood-free, chemically defined medium could be used for lentivirus (LV) production at scale with adherent HEK293T cells, production parameters were first optimized in flatware. Second generation pseudotyped LV was produced in OptiPEAK HEK293t medium, and compared to DMEM+10% fetal bovine serum (FBS), which is traditionally used for adherent HEK293T.
Poster provided courtesy of Pall Corporation.