Efficient, Large-Scale Transfection Of T-Cells Using Flowfect® Technology

Transfection is an invaluable tool used in cell engineering applications to study and modify gene expression by introducing mRNA or DNA into a recipient cell. Traditional transfection methods, such as static electroporation, typically result in adverse effects to cell viability, delivery efficiency, and subsequent cell growth. Kytopen’s Flowfect^®Tx is an automated, closed, benchtop transfection system that enables the delivery of genetic payloads to cells through electromechanical continuous flow.

The objective of this study was to test transfection of T-cells with GFP mRNA for evaluation of cell viability, transfection efficiency, and post-transfection growth using Kytopen’s Flowfect^® technology. GFP-labeled cells were analyzed using flow cytometry methods as a measure of transfection efficiency.