DMSO-Free Cryopreservation Of Mesenchymal Stem Cells In Chemically Defined Systems

As stem cell-based therapies continue to be developed and make up an ever-growing role in medicine, it is essential that the final cell product being administered to the patient is able to ensure both efficacy and safety. To mitigate the potential complications associated with the inclusion of DMSO in final formulations, we developed a DMSO-free cryopreservation solution to avoid the adverse challenges introduced by DMSO and to reduce potential toxicity effects attributed to DMSO. Our solution has been shown to maintain critical morphology and phenotypic characteristics of the cell product, while delivering improvements to cell product recovery yields compared to DMSO containing cryopreservation methods.

Here we feature the materials used, and our protocols, which include freezing and thawing. Discover how mesenchymal stem cells were expanded in chemically defined, serum-free conditions.