DMSO-Free Cryopreservation Of Human T Lymphocytes In Chemically Defined Systems

Engineered human T lymphocytes are commonly cryopreserved before patient administration. Maintaining the integrity and potency of the cells during the cryopreservation process is essential for product efficacy and safety.

There are well-documented significant side effects associated with the DMSO use that can result in adverse reactions in patients, as well as inhibit T cell activation and functionality. Because of this, we have developed a DMSO-free cryopreservation media to provide optimal conditions for cryopreservation without the need or risks of DMSO. Not only does our cryopreservation media provide high viability and improved cell proliferation in chemically defined conditions, it has been shown to improve the recovery of viable cells post-thaw.

It can be used throughout the cell therapy manufacturing process, showing improved and more consistent performance with both starting PBMC material as well as the final expanded and/or modified cell product. Explore our protocols, which include freezing fresh PBMC material, expanding cryopreserved apheresis material, and cryopreservation of expanded T lymphocytes.