Developing A Complete, Scalable Protocol For T Cell Activation And Expansion

By Weiping Sun, Xing Zhang, Zhaolu Yan, Chen Yu, Spencer Chiang, Tianfu Zhang, ACROBiosystems

The development of effective immunotherapy relies on the ability to activate and expand T cells to therapeutic levels. This poster presents a detailed protocol designed to overcome challenges in T cell manufacturing, offering a scalable solution for clinical applications. ​The workflow described in the poster ensures safety, consistency, and robust expansion of T cells by utilizing GMP-grade reagents and serum-free media.

Over a 14-day culture period, the protocol achieves significant cell proliferation, high viability, and favorable memory phenotypes, essential for sustained therapeutic efficacy. Additionally, the process incorporates monitoring tools to track key markers and cell types, ensuring optimal outcomes at every stage. ​

Whether you're working with PBMCs or specific T cell populations, this protocol provides a reliable foundation for advancing immunotherapy research and production. Dive deeper into this innovative workflow to enhance your T cell activation and expansion strategies.