Determining RNA Integrity And Purity By Capillary Gel Electrophoresis

RNA is inherently unstable, so it is susceptible to degradation during manufacture and storage. To ensure the quality of mRNA products, it is important to confirm the integrity of the RNA in terms of its size and length. A high-resolution analytical method is required to separate and quantify the full-length mRNA relative to any fragments that may be present in the sample.

Capillary Gel Electrophoresis (CGE) is commonly employed for the analysis of RNA. Compared to traditional gel-based electrophoresis, CGE offers higher resolution separation, greater reproducibility, and improved quantitation. It also benefits from low sample consumption.

Examine the results of a study challenging the performance of the CGE method in assessing mRNA integrity and purity.