Designing A Plasmid DNA Downstream Purification Process For Production Of Viral Vector, mRNA, And Vaccine Therapies

To benefit as many patients as possible, workflows used for manufacture of mRNA plasmid-based DNA vaccines must achieve greater efficiency. A critical step in the process is production of plasmid DNA (pDNA), which traditionally, delivers low yields and requires complex purification schemes. Plasmid DNA (pDNA) is an essential component of, and key technology for production of viral vector, mRNA, and vaccine therapies.

To meet this growing demand, upstream productivity must be increased to achieve yield and efficiency goals, ensure robust impurity removal and maximize downstream recovery, all while ensuring patient safety.

pDNA manufacturing presents several challenges. This ebook provides you with guidance for your plasmid DNA downstream process development, in addition we explore cell harvest, lysis, neutralization and clarification; chromatographic purification; TFF; and sterile filtration unit operations.