Defining Specificity Within The ELISpot Assay

The Enzyme-Linked Immunosorbent Spot (ELISpot) assay is a foundational technique for quantifying antigen-specific T cells, offering high sensitivity to measure a wide range of cellular immune responses in both human and murine studies. To leverage the full potential of ELISpot as a direct measure of T cell activation, rigorous validation and proper controls are essential for verifying the specificity of the response.

Learn how the ELISpot assay provides exceptional insights into T cell responsiveness, including its superior sensitivity compared to alternative assays. Understanding the critical experiments required to establish specificity is paramount for robust assay development. Discover various options for measuring and confirming response specificity, along with best practices for interpreting assay results relative to your antigen of interest.