ddPCR™ Genome Edit Detection

ddPCR Genome Detection Assays offer a fast, precise, simple, and cost-effective method for detection of genome editing events. Assays can be obtained for any target using Bio-Rad’s easy-to-use, intuitive Digital Assay Site.

ddPCR Genome Edit Detection Assays:

• Enable rapid assessment of HDR and NHEJ edits generated by CRISPR-Cas9 or other genome editing tools
• Detect editing events present at frequencies of ≤0.5%
• Provide absolute quantification of genome editing events from as little as 5 ng  of total gDNA (cells from single well of a 96 well plate)
• Distinguish between homozygous and heterozygous edits in clonal populations
• Results are easily analyzed using QuantaSoft™ Analysis Pro Software