12-Day Stability Of PRIME-XV T Cell Expansion Supplemented With CTGrade GMP Cytokines

When conducting T-cell expansion protocols using systems like the Quantum hollow fiber bioreactor, cell culture media supplemented with interleukins may be stored at room temperature for extended periods, raising concerns about media stability and functionality.

This application note details a study evaluating the stability of PRIME-XV T Cell Expansion XSFM and PRIME-XV T Cell CDM, supplemented with CTGrade GMP Recombinant Human Proteins (IL-2_c126s or a combination of IL-7 and IL-15), when kept at room temperature and protected from light for up to 12 days. The results demonstrate that media stored for this duration did not suffer a significant decrease in function compared to the cold-stored control. The key finding is that the supplemented PRIME-XV media retained full functionality, supporting T-cell expansion protocols that require extended room temperature media storage.

Download the full application note for comprehensive data on cell counts, viability, and CD⁴⁺ : CD⁸⁺ ratios.