3D Cell Culture Handbook - Create More Physiological Relevant 3D Cell Models

Cells and tissues that are cultured in a 3D environment show great potential to advance our understanding of complex biological processes. Organoids and spheroids are increasingly being used in many research applications, including drug discovery, toxicology, disease modeling, and regenerative medicine. These models offer an opportunity to better understand complex biology in a physiologically relevant context where 2D models have not proven as successful. This handbook provides an overview of the cell lines, culture conditions, and analysis techniques that are being used in the transition from 2D to 3D cell models.