Crude Or Purified, Empty Or Full—All-In-One ddPCR Assay For In-Depth AAV Characterization

Measuring the empty-to-full capsid ratio is a cornerstone of gene therapy quality control, yet traditional analytical methods often demand significant time and sample volume. This presentation explores a modern alternative that utilizes droplet digital technology to deliver absolute quantification of AAV vectors. By integrating immunochemistry with PCR sensitivity, this approach allows for the simultaneous detection of capsid and genome titers within a single assay, compatible with both crude and purified samples.

The workflow is designed for efficiency, featuring a four-step process—exogenous DNA removal, binding and ligation, droplet generation, and analysis—that requires minimal manual intervention. Unlike mass-based techniques that may miscount encapsulated contaminant DNA, this sequence-specific detection ensures a more accurate categorization of therapeutic capsids. With a total time-to-result of under eight hours, this solution simplifies complex AAV development tasks into a manageable, high-precision routine. Watch this video to see how an all-in-one digital assay can bring greater clarity and speed to your viral vector production.