CRISPR Gene Editing Workflow

The power to edit a gene is the power to change its function, and with it the biology of a cell. From generating novel cell lines and better animal models for the discovery and preclinical phases of therapeutic research to actually creating a therapeutic itself, such as CAR T-cell therapies, CRISPR gene editing is allowing science to advance rapidly. The downstream applications are countless and are changing the way we perform R&D and the way we think about treating and curing disease.

We present a workflow that guides you through the four main steps of gene editing using the cancer cell line PC3. We designed a gene knockout strategy that uses knock-in of GFP and the puromycin resistance gene to disrupt ICAM-1, a gene known to play a role in cell adhesion and cell signaling. GFP and puromycin resistance serve as easy to follow markers for successful gene disruption. Although we chose a knock-in strategy, this workflow can be applied to most other gene editing experiments. Follow us through the generation and downstream analysis of ICAM-1 knockout cell lines and see how Bio-Rad products support and accelerate gene editing at each stage.