Clean Blood & Tissue DNA Kit
Source: INTEGRA Biosciences
Magnetic bead-based genomic DNA extraction kit.
A better way to extract genomic DNA
Spin column workflows require centrifugation between steps, multiple tube transfers, and can be difficult to automate. The Clean Blood & Tissue DNA Kit uses magnetic beads instead of columns to isolate high purity genomic DNA (gDNA) from blood, buffy coat, tissue and cultured cells – without centrifugation. The kit is compatible with manual workflows and INTEGRA automation for hands-free processing.
How it works
The Clean Blood & Tissue DNA Kit follows a 4 step workflow from sample to purified gDNA.
- Lysis: The sample is treated with a lysis buffer to break open cells, release nucleic acids and denature proteins and other impurities. Heating optimizes sample lysis.
- Binding: Magnetic beads are added to the lysate, where gDNA selectively binds to the bead surface in the presence of a binding buffer.
- Washing: Several wash steps remove proteins, salts and other contaminants, ensuring high gDNA purity.
- Elution: The purified gDNA is released from the magnetic beads into an elution buffer, ready for downstream use. Heating enhances sample elution.