CHOgro Expression System
The CHOgro Expression System is an optimized platform for transient, high titer protein production in suspension CHO derived cells.
This system consists of CHOgro Expression Medium, L-Glutamine and Poloxamer 188 medium supplements, TransIT-PRO® Transfection Reagent and CHOgro Complex Formation Solution. CHOgro® Expression Medium is a chemically defined, hydrolysate-free and animal-origin-free medium.
CHOgro is formulated to provide high density cell growth, and many suspension CHO cells (e.g. Freestyle™ CHO-S) can easily and quickly grow in CHOgro Medium with minimal adaptation. TransIT-PRO Transfection Reagent was developed by empirically testing proprietary lipid and polymer libraries for high transfection performance in suspension CHO and 293 cell types. It is free of animal-derived components and manufactured at Mirus Bio LLC in Madison, Wisconsin, USA.
The combination of CHOgro Expression Medium and TransIT-PRO Transfection Reagent enables robust cell growth and high efficiency transfection that streamlines the transient protein expression process. With this platform, high yields of therapeutic candidates for preclinical studies are achieved.
- Efficient – Enables high protein titers with simple workflow
- Convenient – Quick adaptation to CHO cell lineages
- Optimized – High density growth with minimal cell clumping post transfection
- Worry-free – No commercial license required; animal origin free
The CHOgro Expression System was developed through systematic optimization of transfection protocol parameters including: cell density, transfection reagent, media formulation and culture temperature (learn more about how this system was developed (PDF)). With the CHOgro Expression System, high protein titers can now be achieved in suspension CHO cells through high density transient transfection.