Characterizing AAV Payload With GMP-Compliant svAUC

Accurately characterizing AAV vector payload is essential for ensuring product potency, manufacturing consistency, and regulatory readiness. However, conventional analytical methods often provide only a partial view of capsid populations.

Solvias partnered with a biotechnology company to implement a GMP-compliant sedimentation velocity analytical ultracentrifugation (svAUC) workflow that accurately quantified empty, partially filled, full, and overfilled capsids in a single experiment.

The approach delivered comprehensive, regulatory-ready characterization while preserving native sample conditions and supporting process development, characterization, and batch release.