CD8+ Single Positive T Cell Differentiation From HiPSC With A Feeder-Free System In Vitro

By Lianbin Chen, Xing Zhang, Zhaolu Yan, Na Zhang, Kun Shi, Yuhui Cao, Spencer Chiang, Haonan Li, Tianfu Zhang, ACROBiosystems

T cells play a pivotal role in adaptive immunity, with cytotoxic T cells specifically targeting and eliminating harmful cells. ​ Leveraging human induced pluripotent stem cells (iPSCs) for T cell differentiation offers a transformative approach to optimize therapies for cancer, autoimmune diseases, and other conditions. ​ This innovative feeder-free system simulates the natural differentiation process, enabling the generation of CD8+ single positive T cells in vitro.

By eliminating the need for feeder cells, this method enhances efficiency, scalability, and safety, making it ideal for clinical applications. ​ Key steps include iPSC expansion, differentiation into hematopoietic stem and progenitor cells (HSPCs), and maturation into cytotoxic T cells using recombinant proteins and chemical molecules. ​ This approach holds immense potential for automated manufacturing systems and off-the-shelf cell therapies.

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