Building Robust Potency Assays With In-Cell Western™ Assay Protein Expression Readouts

Potency testing remains one of the most important and challenging aspects of cell and gene therapy development. While mechanism-of-action–based assays are often preferred, the complexity of many advanced therapies can make direct functional measurements difficult to implement consistently. Quantitative protein expression analysis offers an alternative approach for evaluating biological activity and monitoring critical cellular responses linked to therapeutic performance.

The In-Cell Western™ Assay enables multiplexed measurement of intracellular and cell-surface proteins directly in cultured cells, providing sensitive and reproducible protein-based readouts. Combined with effective normalization strategies, these measurements can help establish robust potency assays that support product characterization, process optimization, and quality control.

Learn how quantitative, cell-based protein analysis can strengthen potency assay development and provide reliable insights throughout the cell and gene therapy development and manufacturing lifecycle.