Automated NGS Nextera 16S Metagenomic Sequencing Library Preparation Using The ASSIST PLUS Pipetting Robot

Automating 16S metagenomic library preparation removes much of the complexity that typically comes with amplicon‑based microbiome sequencing. Learn about a streamlined workflow that amplifies the V3–V4 regions of the 16S rRNA gene, integrates indexing for multiplexing, and uses magnetic‑bead purification to deliver clean, sequencing‑ready libraries. Each stage—from PCR setup to dual clean‑up steps—is supported with precise deck layouts, reagent volumes, and run‑ready templates that help reduce errors and standardize output across multiple batches. With hands‑free processing for up to 24 samples, the workflow is built for labs aiming to increase throughput without sacrificing accuracy or reproducibility.

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