By Randall Alfano, Sofia Pezoa, Atherly Pennybaker, and Nathan Hazi
In this poster we demonstrate that the Aber Futurau biomass probe can be used to accurately determine optimal transfection cell density, as cell density at the time of transfection (TF) is a major determinant of overall vector productivity:
- Over/under confluent cultures can yield poor productivity
- Nuclei-based counting is time consuming and reproducibility is limited
We also explore the practical application of biomass probes to maximize vector production and reproducibility in Pall’s iCELLis bioreactor.
Poster provided courtesy of Pall Corporation.