Analysis Of Viral Proteins, Nucleic Acids And Biomolecular Interactions With Light Scattering

Characterizing viral proteins and their interactions with receptors and engineered monoclonal antibodies (mAbs) is key to fundamental understanding of how a virus infects cells and ultimately to the successful development of protein-based drugs. Light-scattering based methods provide essential solutions to addressing these questions. Size-exclusion chromatography coupled to multi-angle light-scattering (SEC-MALS) delivers crucial information regarding sample quality and homogeneity, degree of glycosylation and complex formation. Composition-gradient light scattering (CG-MALS), which does not involve separation, is a label-free method for analyzing binding affinities between molecules in solution and provides detailed information about the nature of homo- and hetero-oligomers.