Addressing Cell Line Development Bottlenecks Progressing From Conditioned Medium To InstiGRO Reagents

The development of cell lines to produce protein biologics and gene therapies has historically been a significant challenge due to the numerous cell culture bottlenecks. From the harsh conditions associated with cryopreservation and transfection to single-cell isolation for regulatory approval to expansion to shaking conditions for bulk manufacture, the health of the cells involved can often take a substantial hit. Whether it be Chinese Hamster Ovary (CHO) cells for the production of protein therapeutics or Human Embryonic Kidney (HEK) cells for the production of new gene therapies, poor viability, and viable cell densities can cause huge workflow inefficiencies. From waiting weeks for cells to recover post-transfection to seeding handfuls of plates to account for poor clonal outgrowth, scientists are constantly being faced with increased project expenditure and timelines due to cell culture limitations.

Whilst Conditioned Medium (CM) has traditionally been used to help improve poor cloning efficiency, the number of clones achieved often fluctuates between projects due to the lack of control over its production. Here, we consider Advanced Instrument’s proprietary InstiGRO™ reagents as a progression from CM with the objective of increasing the number of isolated single cells that successfully transition to form healthy, clonal colonies.