Accelerated Yield Optimization Of Affinity Capture And Polishing Of A Challenging AAV5 Serotype With 96-Well Chromatography Plates And IEX Chromatography

By Julien Buisset*, Rémy Hubert, Alésia Xemaire, Adrien Auffret-Cariou, and Cédrick Rousseaux (*presenting author)

Standard purification approaches do not always translate across AAV serotypes. For AAV5, optimizing affinity chromatography buffer chemistry and pH increased recovery from less than 1% to more than 70%.

Refining anion-exchange chromatography further supported full/empty capsid separation, achieving up to 83.5% full capsids and maintaining performance at 10 L scale. These findings demonstrate how high-throughput 96-well screening can rapidly identify promising conditions while reducing material requirements during process development.

Explore how a serotype-specific approach to affinity capture and polishing can improve recovery, separation, and scalability for challenging AAV purification workflows.