A Simple Flow Cytometry-Based Assay For Detecting CAR Expression Using Fluorokines™

CAR-T cell therapy is a form of cancer immunotherapy that has been remarkably successful for treating certain hematologic cancers. It is based on the principle that the body’s own T cells can be genetically modified to amplify the immune response and effectively eliminate cancer cells. 

One of the current challenges in the field of CAR-T cell therapy is the lack of a simple assay to easily detect and quantify CAR expression following T cell transduction. To address this need, Bio-Techne now offers R&D Systems™ Fluorokines for CAR detection.

Fluorokines™ are a new line of R&D Systems fluorescent-labeled recombinant proteins that allow chimeric antigen receptors (CARs) to be directly stained and detected by flow cytometry. No secondary antibody is needed for Fluorokine™ detection, reducing processing time and eliminating the possibility of background staining that may occur by indirect detection using a secondary antibody. Learn more about how Fluorokines™ are uses to detect CARs by downloading the full application note.