A Novel RNA Lipid Nanoparticle Platform: Gene-Edited CAR T Cells For Off-The-Shelf Cancer Therapy
![GettyImages-1317697233-cancer-t-cells GettyImages-1317697233-cancer-t-cells](https://vertassets.blob.core.windows.net/image/0d201701/0d201701-93a8-4f2b-b9e0-7a4589459215/375_250-gettyimages_1317697233_cancer_t_cells.jpg)
Engineering T cells with expressions of chimeric antigen receptor (CAR) redirect these cells to target tumours, making this a promising cell-based cancer therapy [1]. To engineer universal CAR T cells for allogeneic cell therapy, gene editing approaches [2] can be used to remove risks associated with graft-versus-host disease [3].
Complex gene editing in primary immune can be challenging for some conventional methods [4]. The LNPs use endogenous uptake pathways to deliver therapeutic RNAs, making this technology gentle to the cells.
Here we report on the use of a novel lipid nanoparticle (LNP) reagent in a validated protocol to achieve successful complex gene editing in primary T cells with high efficiency while maintaining high cell viability.
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