A Flow Cytometry Toolkit To Measure Expression Of Metabolic Proteins On Immune Cells

The effect of the glycolytic pathway on T cell activation and cells of the immune system is important for both the understanding of basic immune function and also helping to fuel early immunotherapy discoveries. Just like in other cells in the body, immune cells require energy to carry out their various functions, such as proliferation, cytokine production and antigen presentation.

Most current assays to investigate metabolic pathways in vitro tend to be difficult, expensive and restricted to bulk analysis of heterogeneous populations of cells in culture. While these methods are extremely valuable, they are limited in their ability to illuminate what is happening at the single cell level. John E. Connolly and his team recently developed Met-Flow, a method using monoclonal antibodies and flow cytometry to investigate the metabolic state of individual immune cells by staining rate-limiting targets that are key steps in metabolic pathways. BD Biosciences is excited to enable deeper insights into this growing field by offering a broad collection of conjugated Met-Flow antibodies

The following content demonstrates simultaneous analysis of different metabolic proteins, using validated fluorochrome-conjugated antibodies for flow cytometry. Download now.